Figure 2.

IR laser-induced temperature jump alters SLC26a5-generated NLC and linear capacitance. (A) Under whole-cell voltage clamp, an induced SLC26a5 HEK cell was nominally stepped to the membrane potentials indicated. During the voltage step, an IR laser pulse of 20 ms duration (nominally 40% Capella laser power) was delivered via optical fiber. The laser pulse induced a maximal change in C_m that depended on the holding potential. The change could be either an increase or decrease. (B) C_m-V_m plots of NLC as a function of time after pulse onset. Note the effect on the voltage dependence of NLC, namely, a shift to the right. (C) Changes in V_h and C_lin follow temperature. Rapid shifts and increases in C_lin occur during laser heating and return back to initial levels during bath cooling. Averages are given in the Results section. To see this figure in color, go online.