Figure 5.

Two components of current induced during temperature jump. (A and B) Capacitance increases during temporally incremental IR laser pulses (A) and corresponding currents arise (B). Current magnitude depends on rate of C_m change and duration depends on pulse duration. For all traces, pulses were delivered at a holding potential of 0 mV. (C) Current responses for a 20 ms duration pulse within the holding potential extremes indicated, stepped nominally at 30 mV. Note the fast inward current response during the laser pulse, with a reversal potential predicted to be at positive potentials. Simultaneously, a slow-decaying current with reversal near 0 mV is observed. The red arrow shows the sum of the two components (note step behavior around zero potential that rides on the slower current ramp) and the blue arrow shows the slow component after the fast component ends after the laser pulse. (D) IV plot of average cell currents before and at termination of the IR pulse (mean ± SE; n = 5). The red line is IR-induced current. Note that current voltage dependence is unaffected by temperature, unlike that of NLC.