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. 2013 Jun 28;100(2):252–261. doi: 10.1093/cvr/cvt170

Figure 1.

Figure 1

The absence of blood flow increases endothelial Notch signalling. (A) Vascular anatomy of a Fli1:eGFP transgenic zebrafish (vascular reporter line) for orientation. Scale bar = 500 μm. A boxed region of trunk indicates site of (B) and (C). (B) 3 dpf Tg(CSL-venus)qmc61 transgenic embryos with blood flow (control MO) or no blood flow (tnnt2 MO). Scale bar = 100 µm. Arrowhead indicates dorsal aorta. (C) The mean aortic fluorescence in 3 dpf Tg(CSL-venus)qmc61 embryos control and tnnt2 morphants. Scale bar = 100 µm. (D) 3 dpf Tg(CSL-venus)qmc61 transgenic embryos with blood flow (vehicle treated) or no blood flow (15 mM BDM treatment) from 36 hpf. ISVs, intersegmental vessels. Scale bar = 100 µm. (E) The mean aortic fluorescence in 3 dpf Tg(CSL-venus)qmc61 embryos in vehicle- and BDM-treated groups.