Figure 6.

Intracellular Ca²⁺ levels in SMCs and ECs from the aorta. Intracellular Ca²⁺ levels are expressed as the ratio of the fluorescence intensity (at 510 nm) at alternating 350 (F350) and 380 nm (F380) excitation wavelengths. Please note that corresponding original recordings are presented in see Supplementary material online, Figure S7. (A) Intracellular Ca²⁺ levels in vascular SMCs from Ctr, Trpc3^−/−, and Trpc6^−/− mice under control conditions, in the presence of phenylephrine (PE, 30 µM), and in the presence of PE and 8-Br-cGMP (300 µM). (B) Intracellular Ca²⁺ levels in ECs from Ctr, Trpc3^−/−, and Trpc6^−/− mice under control conditions, in the presence of carbachol (CCh, 30 µM), and in the presence of CCh and 8-Br-cGMP (300 µM). Columns represent means ± SEM. Ca²⁺ levels represent the maximum in the presence of the respective agonist (reached within 2 min) and the minimum in the presence of 8-Br-cGMP (reached within 5 min). Numbers represent the number of cells. Cells were obtained from 5 to 9 mice. Data were analysed using ANOVA followed by the Bonferroni post hoc test. Asterisks on top of the columns represent statistically significant differences of the values in the presence of PE and CCh to the control values, respectively, or of the values in the presence of 8-Br-cGMP to the values in the presence of the respective agonist. ***P < 0.001; n.s., non-significant.