Skip to main content
. 2013 Jul 26;100(2):325–335. doi: 10.1093/cvr/cvt187

Figure 2.

Figure 2

(A) Protocol used in epifluorescence Ca2+ measurements. (Bi) Average trace from last 12 stimulated Ca2+ transients in media (n = 10; grey) or supernatant (n = 14; black); (inset) normalized traces overlayed. (Bii) mean Ca2+ transient amplitude and (iii) decay rate constant. (C) Protocol used in separate epifluorescence experiments. (D) Typical trace of [Ca2+]i during protocol in (C). (Dii) Ca2+ transient parameters and (iii) decay rate constant at low [Ca2+]o (left panel; n = 8) and normal [Ca2+]o (right panel; n = 13). (Ei) Caffeine-induced Ca2+ transient parameters and (ii) decay rate constant at low [Ca2+]o (left panel) and normal [Ca2+]o (right panel). (F) Fura-2AM ratio during SR inhibition with media (n = 10) and supernatant (n = 11). (G) First Ca2+ transient amplitude post-caffeine during protocol C at low [Ca2+]o (left panel) and normal [Ca2+]o (right panel).