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(A) Protocol with 100 nM isoproterenol (ISO) (B) Typical traces of cells superfused with (i) media(n = 13) or (ii) supernatant(n = 12); (iii) Average trace from last 12 transients; media(grey), supernatant(black); (inset) normalized traces overlayed. (Ci–vi) Mean Ca2+ transient parameters. (Di) Number of diastolic Ca2+ releases in supernatant (black) normalized to control (grey); (ii) mean Ca2+ wave frequency and (iii) amplitude.
