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. 2013 Jul 26;100(2):325–335. doi: 10.1093/cvr/cvt187

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© The Author 2013. Published by Oxford University Press on behalf of the European Society of Cardiology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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(A and B) Mean % change in contractile events over media levels in isolated cardiomyocytes (rTbCatL = 2 nM Recombinant T. brucei cathepsin-L + 5 μM dithiothreitol; n = number of cells from number of hearts). (C) Growth curves of biological duplicates (STL349 and STL350) with inducible TbCatL RNAi. (D) Relative TbCatL gene expression to the uninduced culture; triplicate cultures for STL 349 (n = 3) and STL 350 (n = 3). (Ei) % change in spontaneous contractile events in cardiomyocytes for clone STL349. Media [n = 747 (7)], supernatant [n = 817 (7)], uninduced culture [n = 858 (7)], and induced culture [n = 841 (7)]; (ii) % change in spontaneous contractile events in cardiomyocytes for clone STL350. Media [n = 1018 (11)], supernatant [n = 1427(12)], uninduced culture [n = 1597 (12)], or induced culture [n = 1564 (12)].