Figure 2. Identification of PH3⁺ cells in the CPu of the SE brain at 1 h.

Identity of PH3⁺ cells (red) was determined by double immunostaining with cell type specific markers (green). Virtually all the PH3⁺ cells are NeuN⁺ mature neurons (A), not S100β⁺ astrocytes (B), Olig2⁺ oligodendrocytes/oligodendrocyte precursors (C) or Iba1⁺ microglia (D). Arrows indicate PH3⁺/NeuN⁺ (double-labeled) cells and arrowheads indicate PH3⁺ (single-labeled) cells. Single optical confocal microscopy images are shown. Scale bar = 50 μm.