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. 2013 Oct 16;8(10):e75650. doi: 10.1371/journal.pone.0075650

Figure 3. Effects of Golgi glycosylation enzyme inhibitors on LDLr, HMGCoAR and SREBP2 expression in THP-1 macrophages.

Figure 3

THP-1 macrophages were incubated in serum free medium for 24 h at 37°C, and then exposed to glycosylation inhibitors (2.5 ng/ml kifunensine and 2.5 ng/ml swainsonine) in the absence or presence of 40 ng/ml IL-6 or 50 ng/ml TNF-α for 24 h. The cells were harvested and the mRNA expression of SREBP2 and LDLr were determined following the ΔΔ threshold cycle (Ct) protocol for real time RT-PCR as described in the Materials and Methods section. β-Actin served as the reference gene. Results represent the mean± SD from 4 experiments (A). The protein levels were examined by Western blotting (B). The histogram represents mean± SD of the densitometric scans of LDLr, HMGCoAR and N-SREBP2 protein bands from 4 experiments, normalized by comparison with β-Actin and expressed as a percentage of control (C). *P<0.05 vs control; **P<0.01 vs control; #P<0.05 vs IL-6; ##P<0.01 vs IL-6; ΔP<0.05 vs TNF-α; ΔΔP<0.01 vs TNF-α.