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. 2013 Oct 16;8(10):e77719. doi: 10.1371/journal.pone.0077719

Figure 6. Imunoreactivity of MAbs obtained with immunization using Peptide 3.

Figure 6

Reactivity of MAbs raised against Peptide 3 in ELISA using recombinant IL-13RA2-Fc, A; and the synthetic Peptide 3, B. Detection of IL-13RA2, but not of IL-13RA1-Fc with MAbs 1E10B9 and 1E10F9, C. Immunoreactive IL-13RA2 in Western blot of U-251 MG and T98G cell lysates using MAb 1E10B9, D. Expression of IL-13RA2 detected by immunohistochemistry using MAb 1E10B9 in human GBM specimens and normal brain, E, and canine GBMs and normal brain, F.