Figure 1. Anr absences decrease attachment and aggregation in P. extremaustralis.

(A) Attachment to polystyrene plates in 0.5 NE2 medium supplemented with glucose, KNO₃ and casaminoacids. Values represent media ± SD of 5 independent experiments with 12 wells per strain. Different letters showed significant differences among strains (P<0.05) using ANOVA (B). Autoaggregation experiment. The cells were incubated during 2 h without agitation and a culture sample was stained with DAPI. I) wild type strain. II) anr mutant strain. III) anr complemented strain. All observations were performed at 1000X. (C). Slide culture assay to investigate twitching motility. Cells were incubated for 15 h. I) wild type strain. II) anr mutant strain. III) anr complemented strain. Arrows showed rafts in the edge of the culture. All observations were performed using contrast phase microscopy at 400× magnification.