Table 1. Biofilm parameters of wild type and anr mutant of P. extremaustralis. Values represent the mean ± SD of three independent experiments.

Parameters	strain	24 h	48 h	72 h	96 h
Biomass (Bio-volume, µm3. µm−2)	Wild type	17.2±4.4	13.9±3.2	23.2±4.6	14.2±2.5
	anr strain	2.2±0.3*	6.1±2.6*	0.53±0.5*	1.2±0.8*
Average thickness (µm)	Wild type	22.5±7.2	22.3±6.9	34.4±5.5	18.3±3.4
	anr strain	2.5±0.8*	14.7±5.9	1.7±1.5*	2.6±2.0*
Maximum thickness (µm)	Wild type	100.0±26.3	92.0±13.0	81.6±11.6	68.4±20.0
	anr strain	60.5±10.2*	63.7±20.0	65.2±12.7*	70.0±11.6
Roughness coefficient	Wild type	1.4±0.1	1.3±0.3	0.9±0.2	1.1±0.2
	anr strain	1.6±0.4	1.6±0.2	1.7±0.4*	1.6±0.3*

The asterisk (*) denotes significant differences among strains (P<0.05) using the Student’s t test.