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. 2013 Oct 16;8(10):e77508. doi: 10.1371/journal.pone.0077508

Figure 4. Haplotype effects on constitutive and IFNβ-stimulated splicing of IL7Rα.

Figure 4

Freshly purified monocytes (mono), in vitro cultured immature (IL-4, GM.CSF; iDC) and maturing monocyte-derived dendritic cells (IL-4, GM.CSF, LPS; matDC) from healthy control homozygotes of Hap 1 (n = 3), Hap 2 (n = 2) and Hap 4 (n = 2) were incubated +/− IFNβ (1000 IU/ml) for 24 h with triplicates for each individual. Relative expression of membrane-bound (full-length) and ex 6 soluble (sol) IL7Rα isoform was measured as previously described [13] and expressed as a ratio. IFNβ increases the ratio of membrane-bound to ex 6 solIL7Rα receptor isoform expressed in myeloid cells of Hap 2 (Hap 2 iDC, p = 0.026; Hap 2 mono showed a trend, p = 0.056). Mean +/− SEM is shown; *significantly different from media condition; bars represent significant differences between haplotypes in the same subset under the same condition by Student's t test (p<0.05).