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. 2013 Oct 16;8(10):e77508. doi: 10.1371/journal.pone.0077508

Figure 6. TSLP treatment of IFNβ-stimulated dendritic cells induces T cell proliferation in co-culture (A) IFNβ induces expression of tolerogenic genes in dendritic cells.

Figure 6

Expression of IL27p28, IDO and IL10 was measured in in vitro cultured immature (IL-4, GM.CSF; iDC) and maturing monocyte-derived dendritic cells (IL-4, GM.CSF, LPS; matDC) (each in triplicate). Expression was measured by RTPCR relative to GAPDH; mean +/− SEM is shown; *significantly different from media condition by paired t test (IL27p28, p = 0.045; IDO, p = 0.016). (B) IFNβ-treated DCs (IFNβ) reduce proliferation of CD4 T cells compared to untreated DCs (media), but subsequent DC treatment with TSLP partially reverses this effect (IFNβ + TSLP). CFDA-loaded naïve CD4 T cells and antiCD3/antiCD28 beads were added on day 8 to maturing DC culture previously conditioned +/− IFNβ (day 5) and +/− TSLP (day 7). Proliferation of CD4+ cells was assessed on day 13 by reduction in CFDA median fluorescence intensity. The results are representative of two independent experiments.