Table 1. Gene expression in response to IL7Rα signaling in IFNβ-treated DC subsets1.
| IL7 | TSLP | |
| Monocytes | All genes 2: 143 ↑, 67 ↓ | All genes: 215 ↑, 248 ↓ |
| Pathways 3: none identified | Pathways: ↓ immune response9 | |
| Immature DCs | All genes: 79 ↑, 57 ↓ | All genes: 5 ↑, 5 ↓ |
| Pathways: ↑ chemokine activity4 ↑ immune system response5 | Pathways: none identified | |
| Maturing DCs | All genes: 16 ↑, 57 ↓ | All genes: 68 ↑, 61 ↓ |
| Pathways: ↑ up-regulation of cytokine biosynthetic process6 ↓ chemokine activity7 ↓ immune system response8 | Pathways: none identified |
freshly purified monocytes or monocyte-derived DCs were preincubated for 48 h with IFNβ (1000 IU/ml; +LPS for maturing) before 24 h stimulation with IL7 or TSLP (10 ng/ml) and gene expression measured by microarray.
total number of genes upregulated (↑) or downregulated (↓) at least 1.5-fold by IL-7 or TSLP. This data is also presented as a sum of total genes regulated in Figure 5.
gene ontology (GO; http://www.geneontology.org) pathways identified in lists of genes upregulated or downregulated at least 1.5-fold by IL7 or TSLP. Pathway number, names and numbers of differentially regulated members of the pathway and p values, comparing pathway representation in up- or down-regulated gene lists compared to all expressed genes, are given for each condition below.
GO:0008009; LOC728835, CXCL10, CCL4L1, CCL22, CCL17, IL8; (n = 6; p<6×10−7; genes overlapping with list g shown in bold).
GO:0002376; NFκBIA, RGS1, CCL4L1, MMP9, KLF6, CCL17, IL8, LOC728835, CXCL10, FCER1A, CCL22, CCL4L2, CST7, CTLA4; (n = 14; p<2×10−3; genes overlapping with list h underlined).
GO:0042108; CD86, FCER1A; (n = 2; p<0.020).
GO:0008009; CCL2, CCL4, CCL5, CCL18, CCL13, CXCL16, IL8, LOC728835, CCL4L2, CXCL1, CCL8; (n = 11; p<2×10−16; genes overlapping with list d shown in bold).
GO:0002376; CCL2, CCL4L1, MMP9, CXCL16, IL8, LOC728835, LILRA3, TIMP1, IFITM2, CCL5, CCL18, CCL13, HLA-DRA, C1QB, HLA-DPA1, C1QC, CCL4L2, HLA-DRB6, CXCL1, CCL8; (n = 20; p<4×10−14; genes overlapping with list e underlined).
GO:0006955; OAS2, RGS1, IL1RN, LAG3, CD86, IGLL1, CD74, C1QB, IGSF6, C1QC, HLA-DQA1, CXCR4, CLEC7A, LOC554223, HLA-C, RBM4; (n = 16; p<0.048).