Figure 1. Primary and second – tier screening of putative Fusarium oxysporum strain 11C transformants.

Box and Whisker plot depicting the distribution of putative Fusarium oxysporum strain 11C transformants (n =1,563) screened for altered ethanol tolerance during primary screening at 48 hours (A) and 96 hours (B) and transformants (n =402) screened for altered ethanol tolerance during secondary screening (C). Fungi were grown at 25°C in minimal medium [37] with five different treatments; no ethanol control; hygromycinB 60 µg ml^-1 control; 0.5% (vv^-1) ethanol; 6% (vv^-1) ethanol; 10% (vv^-1) ethanol) (Primary-tier) (A, B) or six treatments; no alcohol control; 6% (vv^-1) ethanol; 7% (vv^-1) ethanol; 8% (vv^-1) ethanol; 9% (vv^-1) ethanol; 10% (vv^-1) ethanol (Second-tier) (C). Fungal growth (OD_600nm) was measured after 96 hours using a spectrophotometer (Safire², Tecan, Austria).