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. 2013 Oct 16;8(10):e77501. doi: 10.1371/journal.pone.0077501

Figure 2. Tertiary screening of Fusarium oxysporum strain 11C transformants (n=29).

Figure 2

Fungi were grown in minimal medium [37] supplemented with; no alcohol (A); 2% (vv-1) ethanol (ethyl alcohol; Sigma, UK) (B), 4% (vv-1) ethanol (C), 6% (vv-1) ethanol (D) or 0.25% (vv-1) butanol (n-butanol; Sigma, UK) (E), 0.5% (vv-1) butanol (F) or 0.75% (vv-1) butanol (G) and incubated at 25°C for 7 days. Percentage increase or decrease in fungal growth relative to the positive control (wild-type strain 11C) was determined. Results represent the mean of three independent experiments and bars indicate SEM. Transformants significantly different from the positive control (wild-type strain 11C) are highlighted with an asterisk (level of significance: <0.050 *, <0.01**, <0.001***).