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. 2013 Oct 16;8(10):e76350. doi: 10.1371/journal.pone.0076350

Figure 3. Stimulus-dependent expression of gp96 mRNA in human innate immune cells.

Figure 3

Relative mRNA levels of gp96 are shown (vs. GAPDH/β-actin) in i.v.DCs, i.v.MACs and IMACs from control, UC, and CD patients. Cells were stimulated for 2 h. Results are representative for three experiments. Error bars represent the standard deviation of a single experiment measured in triplicate. (A) Stimulation of i.v.DCs and i.v.MACs with cytokines. (B) Stimulation of IMACs with cytokines. (C) Stimulation of i.v.DCs, i.v.MACs and IMACs with bacterial envelope components. (D) Stimulation of i.v.MACs and IMACs with gp96 protein. c: unstimulated control; TNF: tumour necrosis factor, IFN-γ: interferon-gamma, IL: interleukin IL-12, IL-17, IL-23 (100 ng/mL each); GM-CSF: granulocyte-macrophage-colony stimulating factor (50 ng/mL); IL-4 (5 ng/mL); LPS: lipopolysaccharide, MDP: muramyl-dipeptide (10 ng/mL each); gp96: glycoprotein 96 (10 µg/mL).