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. 2013 Oct 16;8(10):e77487. doi: 10.1371/journal.pone.0077487

Figure 5. Ras1-GTP-Gap1 interaction is essential during pheromone-directed cell elongation.

Figure 5

A, Images of morphological changes upon 10 μM pheromone stimulation. Wild type cells elongate from a single tip, in contrast, the presence of lysed cells (asterix), multiple projection tips (arrows) and enlarged vacuoles (cross) was noted in Ras1 GTPase-defective strains. Scale bar 10 μm. See also Movies S1-4. B-D, Quantification of pheromone-dependent changes in morphology, gene transcription and cell viability following exposure to 10 μM pheromone. E-G, Quantification of pheromone-dependent changes in morphology, gene transcription and cell viability following exposure to 0.1 μM pheromone. All data is mean (±SEM) of three individual experiments.