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. 2013 Oct 16;8(10):e77487. doi: 10.1371/journal.pone.0077487

Figure 6. Ras1-GTP hydrolysis is essential to mediate cell polarity but not MAPK signaling.

Figure 6

A and C, Calcofluor white staining of indicated strains treated with 10 μM pheromone. Scale bar 10 μm. Values shown are percentage loss of cell viability for each population. B and D, Pheromone-dependent transcription as determined using the sxa2>lacZ reporter for the stains indicated. Removal of Scd1 from cells lacking Gap1 prevented pheromone-induced cell death while enabling an elevated transcriptional response. All values are mean of triplicate determinations (±SEM).