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. 2013 Oct 16;8(10):e77487. doi: 10.1371/journal.pone.0077487

Figure 9. Hyperactivation of Ras1 deregulates Cdc42-GTP localization following pheromone stimulation.

Figure 9

CRIB-GFP, a marker for Cdc42-GTP imaged following pheromone stimulation in indicated genotypes, representative images from Movies S5-8. CRIB-GFP is enriched at growing tips in all mitotically growing cells (0 μM). Following 2 h pheromone stimulation CRIB-GFP is enriched at a single tip in wild type cells enabling uni-directional growth to form a polarized conjugation tube. Cells containing hyperactivating Ras1 mutations display CRIB-GFP fluorescence at many discreet locations around the plasma membrane (asterix). These strains fail to coordinate a single site of active Cdc42 and attempt to elongate from multiple sites (arrows). Scale bar 10 μm.