Table 1. Summary of pheromone-induced defects in Ras1-mediated signaling.
|
|
10 μM Pheromone |
||||
|---|---|---|---|---|---|
| Strains |
Cell Death | MAPK | Shmoo | Mating | |
| Wild type | Strain alone | - | ++ | ++ | ++ |
| Δbyr2 | - | - | - | - | |
| Δscd1 | - | ++ | - | - | |
| + pRga4 | - | ++ | ++ | ++ | |
| + pCdc42 | - | + | ++ | + | |
| + pScd1 | ++ | + | + | - | |
| + pPob1 | + | ++ | ++ | ++ | |
| Hyperactive Ras1 mutants | Strain alone | +++ | - | - | - |
| Δbyr2 | - | - | - | - | |
| Δscd1 | - | ++ | - | - | |
| + pRga4 | +++ | - | - | - | |
| + pCdc42 | ++ | + | ++ | + | |
| + pScd1 | +++ | - | - | - | |
| + pPob1 | + | +++ | ++ | ++ | |
Data has been collated from the assays previously described and conducted in this study. Hyperactive Ras1 mutants include fission yeast strains lacking gap1 or containing either Ras1G17V or Ras1Q66L. The extent of the response is represented as follows: +++ increased level of response, ++normal level of response, + reduced level of response and - no response.