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. 2013 Oct 16;8(10):e77484. doi: 10.1371/journal.pone.0077484

Figure 3. Verification of direct miR-525-3p targets using luciferase assays.

Figure 3

(A) Relative luciferase activities after a co-transfection of luciferase constructs and control miRNA or pre-miR-525-3p in EA.hy926 cells. The mean ± s.e.m. of three independent experiments is shown. * indicate significant differences to pmiRGlo transfected cells (* p<0.05, ** p< 0.01). (B) Relative luciferase activities after co-transfection of luciferase constructs and anti-miR-525-3p or control miRNA followed by an irradiation with 2.5 Gy. The Firefly luciferase values were normalized for transfection with Renilla luciferase activity. Relative luciferase activities represent the ratio between normalized luciferase activities of pre-miR-525-3p and control miRNA transfected cells. Grey dashed bars represent sequences with perfect seed sequence matches to miR-525-3p. The mean ± s.e.m. of three independent experiments is shown. * indicate significant differences between control and anti-miR-525-3p transfected cells (* p<0.05, ** p< 0.01). (C) Complementarity of miR-525-3p sequence to the three target genes bearing perfect seed matches. The seed sequence is shown in red. Vertical lines indicate identity between miRNA sequence and corresponding gene sequence.