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. 2013 Oct 16;8(10):e76002. doi: 10.1371/journal.pone.0076002

Figure 1. HIV provirus (A) and APOBEC3G protein (B) levels in blood resting CD4+ T memory lymphocytes.

Figure 1

(A) Blood resting CD4+ T central memory (Tcm) and effector memory (Tem) cells from viremic controller (VC) subjects (n = 7) each had lower HIV provirus copy numbers than those cells from antiretroviral therapy suppressed (AS) non-controller subjects (n = 6). Alu-PCR was used to determine HIV provirus copy numbers, as log integrated copies per million cells. (B) Blood resting CD4+ Tcm and Tem cells from VC subjects (n = 4) each had higher APOBEC3G (A3G) protein levels than those cells from AS non-controller subjects (n = 4). Relative light units (RLU) of A3G bands on immunoblots were quantified using Licor Odyssey, with normalization to β-actin. Cells from which A3G protein levels were determined were from 8 of the same time points studied for each subject in Fig. 1(A) (4 for VC and 4 for AS subjects); protein quantitation was possible for only a subset of the subject cells/time points studied in Fig. 1(A). In (A) and (B), lines indicate median values. P values were determined by 2-tailed Mann-Whitney test.