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. 2013 Oct 16;8(10):e76474. doi: 10.1371/journal.pone.0076474

Figure 5. Methylation profiles around the transcriptional start site of OCTN2.

Figure 5

(A), the genomic sequence spanning −325 to −92 bp in promoter Region-1 of OCTN2. Eighteen CpG sites were located and marked. (B), bisulfite genomic sequencing analysis of Region-1 of OCTN2 in HepG2 cells. Bisulfite modified DNA was amplified by BSP as described under Materials and methods and then sequenced. Pointed star represents methylated CpG sites. (C), DNA methylation profiles of individual methylated CpG dinucleotides in four cancer cell lines. After BSP, five clones were randomly selected and sequenced. The methylated sequences were checked for alignment using MethBLAST. The open and closed circles represent unmethylated or methylated cytosines, respectively.