Figure 1. Functional analysis of the antimorphic human Cry1 expression vector.

(A) Graphic illustration of the pCAG-intron-hCry1^AP.SV40-Neo expression vector. Expression of the antimorphic human Cry1 (hCry1^AP) is driven by the CMV enhancer chicken beta-actin (CAG) chimeric promoter and terminated with the bovine growth hormone polyadenylation site (bGHpA). A selection cassette consisting of a Simian virus 40 (SV40) promoter, a Neomycin (Neo) gene and SV40 polyadenylation signal is placed downstream. (B) Quantitative RT-PCR analysis of hCry1^AP expression in transgenic Bama-minipigs fetal fibroblasts (BM-PFFs). Reverse transcribed total RNA was used for amplification with hCry1-specific exon-exon primers and normalized to endogenous GAPDH. The depicted expression levels are relative to cell clone # 17. The experiment is performed in triplicate and data are presented as mean values ± standard deviation.