FIGURE 5.

RRS1^Col contributes to EDS1-dependent gene expression changes in RPS4^Col auto-immunity. Gene expression microarray analysis was performed on leaf RNAs of 3.5-week-old 35S:RPS4-HS transgenic plants in the Col-0, rrs1-11, or eds1-2 backgrounds at 0, 2, 8, and 24 h after temperature shift. (A) Induced or repressed genes (q-values < 0.01 and >2-fold changes) in 35S:RPS4-HS Col-0 upon temperature shift at any time point and the log₂ ratios compared to 0 h are plotted. Linear regression lines indicate log₂ ratios in 35S:RPS4-HS Col-0 (red) and the 35S:RPS4-HS rrs1-11 or 35S:RPS4-HS eds1-2 mutant lines (black). (B) Log₂ gene expression ratios at 8 h after temperature shift compared to 0 h in 35S:RPS4-HS Col-0 plants for previously identified Pst/AvrRps4-triggered EDS1-dependent genes, shown by Heatmap clustering analysis. (C) Heatmap clustering of 250 genes whose expression changes are RRS1-dependent in 35S:RPS4-HS after temperature shift at any time point (q-values < 0.01 and >2-fold change). Expression patterns for the 250 genes in 35S:RPS4-HS Col-0, 35S:RPS4-HS rrs1-11, and 35S:RPS4-HS eds1-2 lines are shown at 2, 8, and 24 h. Highlighted clusters 1–4 are described in the text. (D) GO term analysis of the 250 RRS1-dependent genes.