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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Neurochem Res. 2013 Aug 6;38(10):2148–2159. doi: 10.1007/s11064-013-1123-z

Fig. 4.

Fig. 4

Expression of NOX isoforms in human astrocytes. After treatment total RNA (1 µg) collected from astrocyte cultures in 12-well plates (106 cells/well, 600 µl DMEM) was treated with DNase and reverse transcribed to cDNA followed by qPCR for NOX isoforms and housekeeping gene HPRT. PCR products were electrophoresed in 4% NuSieve 3:1 agarose gel and stained with ethidium bromide. Data presented are representative of three separated experiments using astrocytes derived from different brain tissue specimens.