Fig. 6.

Effects of IL-1β and IFN-γ on antioxidant enzymes in human astrocytes. Total RNA (1 µg) or cell culture lysates collected from human astrocyte cultures in 12-well plates (10⁶ cells/well, 600 µl DMEM) untreated or treated with IL-1β (10 ng/ml) ± IFN-γ (200 U/ml) for 24h were DNase treated and reverse transcribed to cDNA followed by qPCR or electrophoresed, transblotted and probed, respectively, to assess A) SOD2 and B) catalase expression. Densitometric measurement (normalized to β-actin) of the bands was shown. Data presented are mean ± MSE of three separate experiments using astrocytes derived from different brain tissue specimens. *p<0.05 vs. untreated control.