Fig. 3.
The housekeeping gene LDHA amplified from each queried tissue. (a) LDHA amplification varies by stage, suggestive of ontogenetic differences in cell proliferation (Merchant et al., 2009). (b) Subdivided by sex, the dataset shows stage-differential but not sex-differential expression of LDHA, suggesting the utility of the marker as a normalizer of expression data for genes of interest. Means were log2-transformed for ease of visual interpretation. Lines on bars represent standard error. Numbers above bars indicate the number of biological replicates. The black diamond in each figure indicates the developmental stage in which sex is determined. Asterisks represent significance (***: p < 0.001).