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Primers and annealing temperatures used to amplify housekeeping and sex-differentiating genes in American alligators, Alligator mississippiensis
| Locus | Forward | Reverse | Annealing temperature, °C |
|---|---|---|---|
| LDHA | TGTGACTGCAAACTCCAAGC | GCCGCTACCAATAACACGAT | 57.0 |
| FGF9 | CCCAGGAGTGTGTATTCAGAG | CAGCATGTTCCATCCAAGCC | 60.8 |
| FOXL2 | TACTCSTACGTGGCSCTSAT | TTCTCGAACATGTCCTCGCAGG | 57.0 |
