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. Author manuscript; available in PMC: 2014 Aug 6.
Published in final edited form as: Circulation. 2013 Jul 9;128(6):632–642. doi: 10.1161/CIRCULATIONAHA.113.002714

Figure 1.

Figure 1

OS Induces SREBP2 and miR-33 in HUVECs. HUVECs were exposed to a PS, (12 ± 4 dyn/cm2) or OS (1 ± 4 dyn/cm2) for 14 hr. (A) Representative immunoblot for precursor SREBP2 and mature form of SREBP2. (B) Analysis of levels of SREBP2 mRNA and miR-33 by RT-PCR. (C) The mRNA levels of HMG-CoA synthase, HMG-CoA reductase, squalene synthase, and LDLR. (D) The mRNA levels of ABCA1 and ABCG1. (E) Representative immunoblot for ABCA1 and ABCG1. (F) Total cellular cholesterol level (n = 8). (G) Representative immunoblot for precursor SREBP2 and mature form of SREBP2 in HUVECs treated with MβCD, 25-HC (1 µg/mL), or OS plus 25-HC (1 µg/mL). Data are mean ± SEM from at least 3 independent experiments. Student’s t test or Mann-Whitney U test with exact method was used. ‘*’ indicates p < 0.05.