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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Comp Neurol. 2013 Jun 1;521(8):1844–1866. doi: 10.1002/cne.23263

Fig. 5.

Fig. 5

The paraventricular nucleus of the hypothalamus projects bilaterally to CA2. Single injections of 20nl of 1% fluorogold in 0.2M sodium cacodylate buffer were made 1 month prior to removal of brains. A. Injection into the dorsomedial CA2 (0.80, −1.06, −1.73mm (arrowheads delineate the CA2 region). B. Retrogradely labeled neurons in the PVN. C. Injection into the dorsolateral CA2 (1.85, −1.70, −1.85mm; the two arrowheads delineate the CA2 region and the small insert shows the CA2 region Nissl stained section). D. Retrogradely labeled neurons in the PVN with more labeling ipsilaterally than contralaterally. E. Injection of fluorogold tracer into CA1. F. No labeled cells were seen in the PVN after the CA1 tracer. After 4 weeks, the fluorogold was metabolized or had left the retrogradely labeled neurons of CA2 (panel E) or in CA3 (panels A and C), and is diminished at the sites of injection. Scale bars equal 100 μm.