Fig. 6.
The paraventricular nucleus projects to the dorsal CA2 regions bilaterally. A. Unilateral AAV-GFP injection (200nl) into the PVN and surrounding region (higher magnification in B). C. GFP-positive fibers within the contralateral CA2 pyramidal layer. The CA2 regions are delineated by pairs of arrowheads. Note some fibers in the immediately adjacent CA3. D. GFP-positive fibers within the ipsilateral CA2 and immediately adjacent CA3 pyramidal layer. E. Pyramidal neurons stained with DAPI have surrounding GFP fibers. F. The fornix anterior to the PVN injection site contains GFP-positive fibers. However, the fornix posterior to the PVN injection site did not (not shown). Scale bars equal 100 μm in A, C, D, F; 50 μm in B, E.