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. 2013 Jun 5;52(29):7593–7596. doi: 10.1002/anie.201210317

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© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of Creative Commons the Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Chemical reactivity of [Co^IIINTA(His6-GFP)]. NTA beads with immobilized His6-GFP at Co²⁺ and Co³⁺ centers were incubated with imidazole (250 mm), the chelators NTA and EDTA (25 mm), and the reducing agents cysteamine, DTT, TCEP, and ascorbate (1 mm) in combination with 250 mm imidazole for 1 hour and the amount of eluted His6-GFP was measured. Due to the kinetic inertness of the Co³⁺ centers, His6-GFP is not eluted when bound to the beads as [Co^IIINTA(His6-GFP)].