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. 2013 Jun 5;52(29):7593–7596. doi: 10.1002/anie.201210317

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© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of Creative Commons the Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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His6-GFP (20 μm), treated with various amounts of [Co^IINTA] and 10 mm H₂O₂ for 1 hour, was passed through a Ni-NTA column and eluted with a linear imidazole gradient, and then the GFP fluorescence in each fraction was measured. His6-GFP conjugates with one (b) or multiple Co^III NTA units (a) could be separated from the His6-GFP (c).