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. 2013 Jun 5;52(29):7593–7596. doi: 10.1002/anie.201210317

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© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of Creative Commons the Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Scheme 1 — Using Co³⁺ as the mediator ion between NTA and His6-tagged proteins instead of the commonly used Co²⁺ and Ni²⁺ ions leads to the formation a complex that is drastically more stable in such a coordination environment and is inert to ligand-exchange reactions. The Co³⁺ complex can be obtained through the oxidation of the Co²⁺ complex by H₂O₂.