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. 2013 Jun 20;52(30):7709–7713. doi: 10.1002/anie.201303282

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© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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NMR analyses of reactions catalysed by JmjC demethylase. a) ¹H NMR spectra of JMJD2E-catalyzed formation of acetaldehyde by reaction with ART-Lys(Me₃)-QTAR-Lys(Me/Et)-STGGKA. b) ¹H NMR spectra of JMJD2E-catalyzed formation of acetone by reaction with ART-Lys(Me₃)-QTAR-Lys(iPr)-STGGKA. c) ¹H-¹H COSY and TOCSY spectra of a sample containing ART-Lys(Me₃)-QTAR-Lys(Me/iPr)-STGGKA and JMJD2E after 1 hour of reaction. Correlations corresponding to the hydroxylated lysine fragment are highlighted.