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. 2013 Aug 19;2(10):1049–1056. doi: 10.1242/bio.20135934

Fig. 4. The effects of β-catenin stimulation on pluripotency and lineage marker gene expression in wild-type (E14Tg2A) and Nanog−/− (44Cre6) cells.

Fig. 4.

Wild-type (red bars) and Nanog−/− (blue bars) mES cells maintained in 2i+LIF on gelatin-coated tissue culture plastic were transferred to serum+LIF to allow a proportion of cells to exit pluripotency (a percentage of mES cells differentiate spontaneously in serum+LIF), in the presence or absence of the β-catenin agonist Chiron. The effects of Chiron stimulation of β-catenin on expression of markers of pluripotency (Rex1, Nanog and Oct4), epiblast (Fgf5), neuroectoderm (Sox1 and Zfp521), mesendoderm (T/Bra) and primitive endoderm (Gata6) were then assessed by qPCR after two and four days. Plotted are the levels of individual genes in each sample relative to the levels of the reference gene Gapdh. Error bars above and below each bar represent the standard deviation of the mean of at least 3 qPCR replicates. DMSO is used a control in this experiment as Chiron is dissolved in DMSO.