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. 2013 Aug 14;2(10):998–1006. doi: 10.1242/bio.20135298

Fig. 6. Pex11pβ interacts with DLP1 via Mff.

Fig. 6.

(A) After 48 h treatment with control dsRNA or MFF #2 dsRNA, HeLa cells were transfected for 24 h with FLAG-PEX11β, HA2-DLP1, HA2-MFF, or HA2-MFFR. Cells were then treated with 1 mM DSP, lysed and subjected to immunoprecipitation using anti-FLAG IgG-conjugated agarose. Immunoprecipitates were analyzed by SDS-PAGE and immunoblotting with antibodies to HA, Mff, and FLAG. Arrowhead designates HA2-Mff or HA2-MffR. Input (10%) was loaded in lanes 1–5. (B) HeLa cells treated for 48 h with control dsRNA or two different dsRNAs (FIS1 #1 and FIS1 #2) were transfected for 24 h with FLAG-PEX11β and HA2-DLP1. Cells were treated with 1 mM DSP and analyzed as in panel A, except that antibodies to HA, Mff, FLAG, and Fis1 were used. Lanes 1–5, input (10%). (C) HeLa cells treated for 48 h with MFF #2 dsRNAs were transfected for 24 h with HA2-MFFR, and stained with antibodies to Pex14p (a–c) and Mff (d–f). Scale bar: 10 µm. Insets, higher magnification images of the boxed regions, scale bar: 2 µm. Note that peroxisome morphology was restored by the expression of HA2-MFFR.