Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Oct 14;203(1):129–147. doi: 10.1083/jcb.201212024

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Breslow et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Nups are not detected near the base of the cilium. (A) IMCD3 cells were stained with anti-Nup monoclonal antibody mAb414 and an anti-ninein antibody to mark the base of the cilium. Insets show enlarged view of cilium, and arrowheads point to the base of the cilium, where no mAb414 staining is seen. (B) Nup133, Nup35, and Nup37 fused to GFP were transfected into IMCD3 cells for 48 h before processing for immunofluorescence. (right) All tested Nups localize efficiently to NPCs, as seen by spotted circles in midnuclear focal sections. (left) The centrioles and basal bodies were visualized with ninein and cilia stained with acetylated tubulin. Insets show enlarged views of cilia, and arrowheads point to the base of cilia, where no concentration of GFP signal is observed (middle). Bars: (main images) 5 µm; (insets) 1 µm.