Figure 4.
N-terminal truncations of Cdc12 depend on actin and Cdc15 for localization. (A) Cdc12 N-terminal truncations or wt were treated with DMSO or Lat-A for 30 min. Cells with similar SPB distances are marked by open arrowheads for comparison. (B) Localization of Δ503-Cdc12 to the contractile ring/forming ring (arrows) or clump (arrowhead) in wt or cdc15-140 after growth at 36°C for 4 h and then imaged at 36°C. Bars, 5 µm.