Skip to main content
. 2013 Oct 17;9(10):e1003893. doi: 10.1371/journal.pgen.1003893

Figure 4. PANΔENE is stabilized and hyperadenylated upon exosome depletion.

Figure 4

(A) Western blot analysis of cells transfected with either a non-targeting control siRNA or siRNA pools against RRP6, DIS3, or both. Blots were probed with antibodies specific for RRP6, DIS3, and β-actin (loading control). (B) Top, Representative transcription pulse assay from cells transfected with the indicated siRNAs. The same blot and exposure are shown for siControl and siRRP6/DIS3. Lower panels, Overexposed version of the blot and 7SK loading control. (C) Decay curves of the transcription pulse assays as shown in (B)(n = 3). (D) Northern blot showing the mobility of PANΔENE RNA from RRP6 and DIS3 depleted cells before and after treatment with RNase H and oligo(dT).