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. 2013 Oct 17;9(10):e1003893. doi: 10.1371/journal.pgen.1003893

Figure 5. Intronless β-globin decay requires canonical PAP and exosome activity.

Figure 5

(A and B) Representative blot of a transcriptional pulse assay with βΔ1,2 or βΔ1 reporters from cells transfected with the indicated siRNAs. Right, Samples from siRRP6/DIS3 lanes were treated with RNase H and oligo (dT). Single asterisk marks the β-globin pre-mRNA and double asterisk denotes the extremely hyperadenylated RNAs. (C) Decay curves of the intronless β-globin reporter data from cells transfected with the indicated siRNAs (n = 3). The siDIS3/siRRP6 data were not well fit by two-component exponential decay regressions and are represented as linear interpolations (D) Results from a transcription pulse assay of βΔ1,2 from cells transfected with the indicated siRNAs. Blot was overexposed to reveal the extremely hyperadenylated RNAs (double asterisks). The relative mobility and sizes of the large and small ribosomal subunits are indicated on the right.