Figure 6. Endogenous transcripts are subject to PABPN1-mediated hyperadenylation.

(A) Schematic diagram of the in vivo labeling procedure. See text for details. (B) Bulk poly(A) tail analysis of labeled RNAs from cells transfected with indicated siRNAs. Prior to harvesting the RNA, cells were incubated with EU for 2 hr as indicated. (C) In vivo labeling experiment with cells transfected with the indicated siRNAs. Double asterisk marks extensive hyperadenylation observed upon RRP6 and DIS3 co-depletion. (D) Quantification of the poly(A) tails greater than 400 nt in length from (C). We “boxed” signals corresponding to poly(A) tails >400 nt, subtracted background signal (no EU) and normalized to the signal from the siRNA control samples.