Figure 6. Predicting genus-specific KOs in genera from the HMP tongue dorsum samples.

To restrict our analysis to well-sampled genera, only genera for which at least 10 reference genomes are available and for which at least 5 genus-specific KOs were obtained are considered. The presence (or absence) of genus-specific KOs across the set of sequenced species from each genus is illustrated by the presence (or absence) of a black dot. Gray dots indicate that the KO was present in only a subset of the sequenced strains of that species. KOs predicted to be present by metagenomic deconvolution are shown using colored dots. Results are shown for several regression methods, including least squares (LS, 89% accuracy, 82% recall), non-negative least squares (NNLS, 90% accuracy, 82% recall), and lasso (90% accuracy, 92% recall). See also Supporting Text S1.