Skip to main content
. 2013 Oct 17;9(10):e1003246. doi: 10.1371/journal.pcbi.1003246

Table 1. Experimental validation of the binding events predicted by dPeak analysis of Inline graphic PET ChIP-Seq data.

Genea Predicted position True positionb Distance Primerb Conditionc
yejG 2,276,288 2,276,299 11 P 1 Aerobic
yejG 2,276,432 2,276,419 13 P 2 Aerobic
spr 2,267,945 2,267,942 3 P 1 Aerobic
spr 2,267,825 2,267,833 8 P 2 Aerobic
dcuA 4,364,876 4,364,866 10 P 1 Anaerobic
dcuA 4,364,975 4,364,974 1 P 2 Anaerobic
aroL 405,583 405,579 4 P 1 Anaerobic
aroL 405,489 405,504 15 P 2 Anaerobic
serC 956,823 956,802 21 P 1 Aerobic
serC 956,789 (Not validated) N/A Aerobic
hybO 3,144,382 3,144,385 3 P 1 Anaerobic
hybO 3,144,438 (Not validated) N/A Anaerobic
ybgI 742,036 742,030 6 P 1 Aerobic
ybgI 741,859 741,874d 15 P 1 Aerobic
ptsG 1,157,005 1,156,989 16 P 1 Aerobic
ptsG 1,156,866 1,156,849d 17 P 1 Aerobic
(a)

The genes with promoters harboring the predicted binding events.

(b)

The true positions were determined by primer extension experiments (Figure 5A).

(c)

The conditions under which binding events are validated.

(d)

We report results based on the RegulonDB annotations for ybgI and ptsG genes as the primer extension products for these genes were too large to accurately map with the sequencing ladder.