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. 2013 Oct 17;8(10):e75827. doi: 10.1371/journal.pone.0075827

Table 2. Results of the RLB and modified cox III qPCR assays for selected samples and clones.

Sample RLB result Clone coxIII qPCR result (Tm°C) Phylogenetic classification
HIP A2 Theileria sp. (buffalo), Theileria sp. (buffalo), T. parva
T. buffeli, T. mutans 1.1 *NSP (T. velifera/T. parva) (47.2) T. velifera
1.7 *NSP (T. taurotragi/T.mutans) (62) T. velifera-like
1.8 T. taurotragi (56°C) T. mutans-like A
1.9 Theileria sp. (buffalo) (40.5) Theileria sp. (buffalo)
KNP K8 T. velifera, T. mutans T. parva, T. taurotragi (55.3 )
2.2 *NSP (T. taurotragi/T. mutans) (61.8) T. velifera-like
KNP K4 T. mutans T. parva, T. taurotragi (55.8)
3.4 T. taurotragi (55.5°C) T. mutans-like A
3.8 * NSP (T. taurotragi/T. mutans) (59.3) T. mutans-like B
3.9 * NSP (T. taurotragi/T. mutans) (60.7 ) T. mutans-like C
OVI 8227 T. mutans T. parva, T. mutans
4.7 * NSP (Theileria sp. (buffalo)/T. velifera) (42.8) ** Inconclusive
4.10 * NSP (T. taurotragi/T. mutans) (59) T. mutans-like B
KNP E20 T. velifera, T. mutans T. parva, * NSP (T. taurotragi/T. mutans ) (56.5)
5.10 T. taurotragi (56 ) T. mutans-like A
KNP 102 T. parva T. parva T. parva
6.1 T. parva (48.3 ) T. parva
6.2 T. parva (48 ) T. parva
KNP E10 T. mutans * NSP ( Theileria sp. (buffalo)/ T. velifera ) (42.3), T. taurotragi (55.5)
7.1 * NSP (T. taurotragi/T. mutans) (62 ) T. mutans-like C
KNP E2 T. velifera, T. mutans * NSP (Theileria sp. (buffalo)/ T. velifera )(42), T. taurotragi
8.5 * NSP (T. taurotragi/T. mutans) (61.2 ) T. velifera-like
8.6 T. taurotragi (55.3 ) T. mutans-like A
KNP C8 T. velifera, T. mutans * NSP (T. taurotragi/T. mutans) (62 ), T. parva , T. mutans
9.1 T. mutans (64 ) T. mutans
9.4 * NSP (T. taurotragi/T. mutans) (62 ) T. mutans-like C
KNP B22 T. parva, T.mutans, T.velifera T. parva, Theileria sp. (buffalo)
10.10 T. mutans (63.5 ) T. mutans-like C
Addo 65 T. buffeli T. buffeli (53.2) T. buffeli-like
Addo 66 T. buffeli T. buffeli (53.3) T. buffeli-like
Addo 69 T. buffeli T. buffeli (53.1) T. buffeli-like
Addo 70 T. buffeli T. buffeli (53.7) T. buffeli-like
Addo 73 T. buffeli T. buffeli (53.5) T. buffeli-like
Addo 74 T. buffeli T. buffeli (53.5 ) T. buffeli-like
Addo 76 T. buffeli T. buffeli (53.2) T. buffeli-like
*

Indicates a non-species specific melting peak located between the peaks for the species indicated in brackets.

**

Did not cluster with any of the other sequences and its identity could not be established.