Table 2. Results of the RLB and modified cox III qPCR assays for selected samples and clones.
Sample | RLB result | Clone | coxIII qPCR result (Tm°C) | Phylogenetic classification |
HIP A2 | Theileria sp. (buffalo), | Theileria sp. (buffalo), T. parva | ||
T. buffeli, T. mutans | 1.1 | *NSP (T. velifera/T. parva) (47.2) | T. velifera | |
1.7 | *NSP (T. taurotragi/T.mutans) (62) | T. velifera-like | ||
1.8 | T. taurotragi (56°C) | T. mutans-like A | ||
1.9 | Theileria sp. (buffalo) (40.5) | Theileria sp. (buffalo) | ||
KNP K8 | T. velifera, T. mutans | T. parva, T. taurotragi (55.3 ) | ||
2.2 | *NSP (T. taurotragi/T. mutans) (61.8) | T. velifera-like | ||
KNP K4 | T. mutans | T. parva, T. taurotragi (55.8) | ||
3.4 | T. taurotragi (55.5°C) | T. mutans-like A | ||
3.8 | * NSP (T. taurotragi/T. mutans) (59.3) | T. mutans-like B | ||
3.9 | * NSP (T. taurotragi/T. mutans) (60.7 ) | T. mutans-like C | ||
OVI 8227 | T. mutans | T. parva, T. mutans | ||
4.7 | * NSP (Theileria sp. (buffalo)/T. velifera) (42.8) | ** Inconclusive | ||
4.10 | * NSP (T. taurotragi/T. mutans) (59) | T. mutans-like B | ||
KNP E20 | T. velifera, T. mutans | T. parva, * NSP (T. taurotragi/T. mutans ) (56.5) | ||
5.10 | T. taurotragi (56 ) | T. mutans-like A | ||
KNP 102 | T. parva | T. parva | T. parva | |
6.1 | T. parva (48.3 ) | T. parva | ||
6.2 | T. parva (48 ) | T. parva | ||
KNP E10 | T. mutans | * NSP ( Theileria sp. (buffalo)/ T. velifera ) (42.3), T. taurotragi (55.5) | ||
7.1 | * NSP (T. taurotragi/T. mutans) (62 ) | T. mutans-like C | ||
KNP E2 | T. velifera, T. mutans | * NSP (Theileria sp. (buffalo)/ T. velifera )(42), T. taurotragi | ||
8.5 | * NSP (T. taurotragi/T. mutans) (61.2 ) | T. velifera-like | ||
8.6 | T. taurotragi (55.3 ) | T. mutans-like A | ||
KNP C8 | T. velifera, T. mutans | * NSP (T. taurotragi/T. mutans) (62 ), T. parva , T. mutans | ||
9.1 | T. mutans (64 ) | T. mutans | ||
9.4 | * NSP (T. taurotragi/T. mutans) (62 ) | T. mutans-like C | ||
KNP B22 | T. parva, T.mutans, T.velifera | T. parva, Theileria sp. (buffalo) | ||
10.10 | T. mutans (63.5 ) | T. mutans-like C | ||
Addo 65 | T. buffeli | T. buffeli (53.2) | T. buffeli-like | |
Addo 66 | T. buffeli | T. buffeli (53.3) | T. buffeli-like | |
Addo 69 | T. buffeli | T. buffeli (53.1) | T. buffeli-like | |
Addo 70 | T. buffeli | T. buffeli (53.7) | T. buffeli-like | |
Addo 73 | T. buffeli | T. buffeli (53.5) | T. buffeli-like | |
Addo 74 | T. buffeli | T. buffeli (53.5 ) | T. buffeli-like | |
Addo 76 | T. buffeli | T. buffeli (53.2) | T. buffeli-like |
Indicates a non-species specific melting peak located between the peaks for the species indicated in brackets.
Did not cluster with any of the other sequences and its identity could not be established.