Figure 4. Coverage area of viable hESC colonies and pH of post-culture media under the alkaline or normal media conditions.

(A) The coverage area of viable hESC colonies at 6–30 hours of culture after being normalized over the first time point at 6 hours. Values are mean ± standard error of the mean: n = 6. The hESCs cultured under the alkaline media condition proliferated and grew to confluency, indicating that the initial increase of media pH to 8.1 was not the direct cause of cell death observed in the cultures with the Mg samples. (B) The average pH of post-culture media collected at every 24 hours for 72 hours (*p<0.05 compared to the control). Values are mean ± standard deviation: n = 3.