Figure 7. Media pH and Mg ion concentrations in the post-culture media collected at every 24 hours after hESCs were cultured in the media supplemented with Mg ion dosages.

(A) Average pH and (B) Mg ion concentrations in the post-culture media when the supplemental Mg ion dosages ranged from 0.4–40 mM (*p<0.05 compared to the control at each time point respectively). Values are mean ± standard deviation; n = 3. The cell counts in the hESC cultures supplemented with 30 and 40 mM Mg ions were statistically lower than the cultures with lower Mg ion dosages and the control, and, therefore, less acidic metabolites formed and the pH values were statistically higher. Additionally, statistically greater Mg ion concentrations were observed in the post-culture media at the critical Mg ion concentration of 10 mM and above, as compared with the control, 0.4 mM and 4 mM media conditions at each respective time point.